Data on the epitope mapping of soybean A2 and A3 glycinin

The complete amino acid sequence of the A3 subunit of the glycinin seed storage protein of the soybean (Glycine max (L.) Merrill).

The amino acid sequence of the A3 subunit, an acidic subunit of the glycinin seed storage protein of the soybean (Glycine max (L.) Merrill), has been determined by sequence analysis of the peptides obtained by cyanogen bromide cleavage followed by enzymatic digestion and partial acid hydrolysis. The A3 subunit is composed of 410 amino acids (Mr approximately 46,000). It has been confirmed that ...

Linear IgE-epitope mapping and comparative structural homology modeling of hazelnut and English walnut 11S globulins.

Allergic reactions to walnuts and hazelnuts can be serious. The 11S globulins (legumins) have been identified as important allergens in these and other nuts and seeds. Here we identify the linear IgE-binding epitopes of walnut and hazelnut 11S globulins, and generate 3D 11S globulin models to map the locations of the epitopes for comparison to other allergenic homologues. Linear IgE-epitope map...

Partial Epitope Mapping of Alfalfa mosaic Virus and the Effect of Coat Protein Gene Mutation on Aphid Transmission

Whole-Genome Resequencing Identifies the Molecular Genetic Cause for the Absence of a Gy5 Glycinin Protein in Soybean PI 603408

During ongoing proteomic analysis of the soybean (Glycine max (L.) Merr) germplasm collection, PI 603408 was identified as a landrace whose seeds lack accumulation of one of the major seed storage glycinin protein subunits. Whole genomic resequencing was used to identify a two-base deletion affecting glycinin 5 The newly discovered deletion was confirmed to be causative through immunological, g...

Assessment of seed storage protein composition of six Iranian adopted soybean cultivars [Glycine max (L.) Merrill.]

Seed protein quality is an important topic in the production of soybean. The quality of soybean proteins is limited by anti-nutrient proteins and low levels of essential sulfur amino acids. In this study, protein content and solubility of six cultivars were evaluated and seed storage proteins were analyzed using SDS-PAGE and scanning densitometry. The results showed that seed storage protein ba...